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KMID : 0043320040270121202
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Archives of Pharmacal Research
2004 Volume.27 No. 12 p.1202 ~ p.1206
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Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Lithospermic Acid B in Rat Serum
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Kim Hui-Hyun
Ji Hye-Young
Lee Hye-Won
Kim Youn-Chul
Sohn Dong-Hwan
Lee Hye-Suk
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Abstract
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A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/ MS) method for the determination of lithospermic acid B (LSB) in rat serum was developed. LSB and internal standard, 7-hydroxy-3-phenyl-chromen-4-one (HPC) were extracted from rat serum with methyl-tert-butyl ether at acidic pH and analyzed on a Luna column with the mobile phase of acetonitrile-ammonium formate (10 mM, pH 6.5) (50:50, v/v). The analytes were detected using a negative electrospray ionization tandem mass spectrometry in the multiple- reaction-monitoring mode. The standard curve was linear over the concentration range of 10.0-500 ng/mL. The coefficient of variation and relative error for intra- and interassay at three QC levels were 1.1~6.2% and -10.3~-2.7%, respectively. The recovery of LSB from serum sample ranged from 73.2 to 79.5%, with that of HPC (internal standard) being 75.1 %. The lower limit of quantification for LSB was 10 ng/mL using 50 of serum sample.
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KEYWORD
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Lithospermic acid B, Rat serum, LC-MS/MS
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